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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Discriminating Clonotypes of Influenza A Virus Genes by Nanopore Sequencing
doi: 10.3390/ijms221810069
Figure Lengend Snippet: Comparison of sequencing methods among Illumina DNA and MinION RNA/cDNA. ( a ) Sequence logo for variation sites in three sequencing results. Variation sites were identified based on Illumina sequencing reads. ( b ) Number of separated reads for each segment in three sequencing methods. For Illumina sequencing result, the read number was the normalization of total mapped reads length over consensus sequence length for each segment, respectively. For MinION sequencing results, reads of with lengths greater than 80% of the segment full length were calculated. ( c ) Neighbor-joining tree of both direct RNA and cDNA sequencing reads included long reads (length ≥ 80%) only. Branch of direct RNA sequencing reads were colored in red. ( d , e ) Boxplots for total sequencing errors (mismatch and indels), and mismatch, indels, re-spectively for each segment in MinION direct RNA and cDNA sequencing. ( f , g ) Boxplots for total sequencing errors in MinION direct RNA and cDNA sequencing. Known variation identified by Illumina sequencing were not included. Outliers were not displayed for simplicity.
Article Snippet: Total RNA was extracted with the NP968-C Nucleic Acid Extraction System and
Techniques: Sequencing, RNA Sequencing Assay